云南怒江傈僳族自治州古树名木资源现状调查

对怒江傈僳族自治州人为活动区域古树名木资源现状进行调查。结果表明:共记录到古树名木496株,涉及36科54属76种,其中有8种国家重点保护野生植物,分别为澜沧黄杉、秃杉、南方红豆杉、云南榧树、千果榄仁、红椿、光叶珙桐和香果树;怒江州古树名木主要分布在乡村街道及远郊野外,占总数的98.8%,63.6%的古树生长在海拔1500~1999 m;古树名木的生长环境及长势总体较好,但保护力度相对薄弱,应采取相关措施加强保护和管理。     

九峰科技植物资源现状与保护对策

经过查阅历史档案、询问当事人及外业核查、调查,结果表明:九峰地区现有39块试验现场,面积124.7hm^2;实施各类科技项目53项,项目规模面积140.25hm^2,涉及裸子、被子等植物73科,150属,194种(类),已形成科技植物群体;在九峰森林植物本底调查、林木种质资源收集与保存、林木品种遗传改良及繁殖技术等13个学科方向及近120个植物品种开发研究方面取得了重大突破和创新;鉴于九峰科技植物资源现状,本文提出了相应的保护对策与措施。     

江西省薄壳山核桃产业及苗木生产现状分析

薄壳山核桃树形挺拔、树冠优美,果仁营养丰富,木材质地优良,是一种极具市场竞争力的高效生态经济型木本油料树种;但在江西省还处于初始发展阶段,科研基础薄弱,产业发展相对滞后;本文通过分析江西薄壳山核桃产业发展与苗木生产现状及存在问题,总结提出了今后科研开展的方向及促进产业发展的对策。     

果蔬籽油生物活性研究进展

果蔬籽油是由果蔬籽经压榨或萃取工艺制得的植物油脂。果蔬籽油含有丰富的维生素、多不饱和脂肪酸、有机酸等活性成分,这些成分赋予了果蔬籽油抗氧化、抗癌、调节血脂和调节免疫系统等生物活性。本文综述了果蔬籽油生物活性的研究进展,旨在为果蔬籽油的综合开发和利用提供理论依据和指导。     

广东省农地边际化特征及其影响因素的地理探测

为了明确经济发达地区广东省农地边际化现象及其主要影响因素。本研究采用“单位成本纯收益指标”、“农地利用集约度指标”和“农作物播种面积指标”三大主要指标,对广东省2000—2015年农地边际化特征进行提取。选择影响农地边际化特征的19个因子,利用地理探测器方法对农地边际化现象的核心影响因素进行挖掘。结果表明：2000—2015年广东省共发生3次比较明显的农地边际化现象,分别在2001—2002年、2005—2006年、2010—2013年,且第3次边际化持续时间最长,情况最严重。社会经济发展水平与结构指标和农户家庭资源要素结构变化指标对农地边际化的影响程度较大,其中第三产业产值比重、农村农户固定资产投资、粮食总播种面积对农地边际化现象有较强的解释力,q值分别为0.8067、0.8055、0.7916。任何两因子相互交互作用都能增强对农地边际化现象的解释力。     

AM真菌改善植物磷营养吸收和转运机制的研究进展

旨在深入了解丛枝菌根(Arbuscular mycorrhizae,AM)真菌在植物吸收和转运磷元素方面的机制。本研究归纳了近年来关于AM真菌能够促使植物改善磷营养（如磷酸盐转运蛋白、磷酸酶基因等）相关的最新研究成果,着重分析了AM真菌的菌根吸收途径,总结了国内外关于AM真菌对水溶性无机磷、难溶性无机磷和有机磷等3种土壤磷存在形态下的利用机制。最后指出该领域仍存在的一些问题以及未来的研究侧重点。     

金刺梨黑斑病病原菌的分离及鉴定

金刺梨黑斑病主要于开花期危害幼嫩的果梗,果梗受害后产生黑色干腐病斑,导致整个花序从果梗处脱落。对黑斑病病原菌进行了分离和鉴定,通过形态学特性、致病性测定、rDNA-ITS序列分析,初步确定该病主要由拟茎点霉属(Phomopsis Sacc.)的Phomopsis.capsici真菌引起。     

川黔等西南地区李子主要病虫害及防治技术

李适应性广,耐瘠薄干旱,是四川、重庆等西南山区的特色经济树种。李果实外观亮丽,脆甜爽口、营养丰富,是深受人们喜爱的特色水果之一。近年来四川、贵州、重庆地区青脆李、空心李、蜂糖李等地方品种有了迅速发展,促进了贫困山区脱贫致富和产业结构调整。但由于管理粗放和防治措施不当,致使李子生产区域病虫害发生严重,影响了果实的品质和经济效益。为了促进李子产业化发展,结合主要产区生产实际,总结出西南地区常见的病虫害及其防治措施,为李子生产提供技术参考。     

Isolation and Characterization of GhLEA3 Gene from Upland Cotton and Its Expression in Response to Low Temperature Stress

[Objective] Based on the analysis of cotton GhLEA3 gene structure and its expression model in chilling stress, we investigated the resistance function of GhLEA3 in response to cold stress. [Method] We cloned GhLEA3 from upland cotton by homologous sequence cloning way. We analyzed the properties of the GhLEA3, and constructed phylogenetic tree by bioinformatics analysis. We constructed the transient expression vector 35S∷GhLEA3-GFP by In-Fusion connection technology and studied the subcellular localization of GhLEA3. The expression of GhLEA3 gene in leaves of TM-1 at three-leaf stage under low temperature stress treatments (4 ℃, 24 h) was performed. Agrobacterium tumefaciens mediated floral dipping method was used to transform the gene with expression vector 35S∷GhLEA3-GFP into Arabidopsis thaliana wild type. Low temperature germination experiment at 4 ℃ was conducted to verify germination ability of the transgenic Arabidopsis thaliana T₃ seed. The transgenic Arabidopsis seedlings were treated at 4 ℃(low temperature), and all the leaves were taken to measure their electrical conductivity. [Result] The coding sequence of GhLEA3 gene is 1 218 bp, which encodes 405 amino acids. GhLEA3 protein includes 4 functional domains of PF02987. Phylogenetic analysis showed that the similarity of amino acid sequences between upland cotton GhLEA3 and Arabidopsis thaliana AtLEA3 was 52.6%. GhLEA3 might be mainly localized in vacuoles and small vesicles. GhLEA3 was up-regulated in leaves after low temperature treatment. The germination rate of transgenic Arabidopsis thaliana of T₃ generation at low temperature was significantly higher than that of wild type, and its electrical conductivity of leaves after low temperature treatment was significantly lower than that of wild type. [Conclusion] GhLEA3 belonged to the member of LEA3 family. Phylogenetic analysis showed that GhLEA3 had the closest relationship with AtLEA3. GhLEA3 was induced by low temperature stress and may play an important role in improving cold resistance.     

Bird-flower interactions in an urban area: Ceiba pubiflora provides nectar and promotes biodiversity in the city

Urbanization has negative effects on plant-animal interactions by reducing plant richness and increasing resource limitations. However, it is possible to replant these areas, and plant species can be selected based on the resources they provide, as well as their relationships with animals. I observed several trees of Ceiba pubiflora (Malvaceae, Bombacoideae) to identify the bird species interacting with their flowers in an urban area. This plant may be especially important, as it blossoms during the dry season, when the overall flower availability in the study area is low. It may also be important during a short period of decline in the fleshy fruit supply. Even though C. pubiflora was growing in the city and had a low volume of nectar accumulating in its flowers (mean of 1.73 μl; SD = 1.39 μl), it attracted a variety of birds (25 species belonging to six families), some of which are usually regarded as forest dependent. Nectarivorous birds from the family Trochilidae (hummingbirds, n = 12 species) had the highest percentage of visits (64.08%), especially during periods of relatively high nectar sugar concentration and calorie availability (c. 18% and 1.5 cal., respectively). Hummingbirds primarily acted as nectar thieves but also sometimes as potential flower pollinators. In contrast, several nonnectarivorous birds (frugivore-granivores, frugivore-insectivores and omnivores) acted only as nectar robbers or flower predators. Ceiba pubiflora flowers appear to be an important source of energy for birds in the urban area. The data of this study show that C. pubiflora has utility as management tool to promote and conserve biodiversity in cities.